Induction of G 1 − cells at high frequency
Open Access
- 1 December 1979
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 40 (1) , 33-42
- https://doi.org/10.1242/jcs.40.1.33
Abstract
Experimental conditions are reported on the induction of G1- cells at high frequency by ultraviolet irradiation of temperature-sensitive cells arrested in G1 phase at the restricted temperature. Neither the wild type cells of Balb/C-3T3 nor the temperature-sensitive derivative of t8 A83 grown at the permissive temperature could be ‘mutated’ under similar conditions. One such ‘mutant’ (R18) was ‘inducible’ from a G1+ phenotype at 33 °C to G1- state at 38 °C within one cell generation time. The ‘inducible’ property of R18 from G1+ to G1_ lends support to the theory that the duration of the G1+phenotype is determined by the level of precursors for DNA synthesis. Since the G1- variants were isolated from revertants, the frequency of ‘mutagenesis’ appeared to be artificially high.Keywords
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