Permeability of Nitric Oxide through Lipid Bilayer Membranes
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research
- Vol. 24 (5) , 343-349
- https://doi.org/10.3109/10715769609088032
Abstract
Profiles of the local nitric oxide (*NO) diffusion-concentration product across the egg yolk phospha-tidylcholine membrane in the absence and presence of 30 mol% cholesterol were obtained using line-broadening electron paramagnetic resonance (EPR) and lipid-soluble nitroxide spin labels. Membrane *NO permeability coefficients were calculated from these profiles. At 20°C, values of 93 and 77 cm/s for membranes in the absence and presence of cholesterol were obtained, compared with 73 and 66 cm/s for water layers of the same thickness as the membranes. Fluid-phase membranes are not barriers to *NO transport. Cholesterol significantly increases *NO transport in the center of the lipid bilayer.Keywords
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