Studies of Spin-Trapped Radicals in γ-Irradiated Aqueous Solutions of Thymidine-5′-Monophosphate and Cytidine-5′-Monophosphate by Liquid Chromatography and ESR Spectroscopy
- 1 October 1977
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 72 (1) , 89-99
- https://doi.org/10.2307/3574559
Abstract
Aqueous solutions of TMP and CMP were .gamma.-irradiated with 2-methyl-2-nitrosopropane as a spin-trapping reagent. Liquid chromatography was applied to separate the stable spin-trapped radicals in the irradiated solutions. The eluate was passed through flow cells of ESR and UV detectors and collected by a fraction collector. The amount of residual sugar in each fraction was determined by the phenol-H2SO4 method. From the combined results of the ESR, UV and sugar determinations, the structures of most of the trapped radicals were identified. The short-lived radicals which were trapped by 2-methyl-2-nitrosopropane were radicals on N-1, C-5 and N-3 positions of the base in the TMP and C-5 and C-6 base radicals in the CMP.This publication has 1 reference indexed in Scilit:
- Studies of Short-lived Radicals in the γ-irradiated Aqueous Solution of Uridine-5′-monophosphate by the Spin-trapping Method and the Liquid ChromatographyInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1976