Studies on the Diversity of Inositol-Containing Yeast Phospholipids: Incorporation of 2-Deoxyglucose into Lipid
- 1 January 1972
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 109 (1) , 81-88
- https://doi.org/10.1128/jb.109.1.81-88.1972
Abstract
Seven inositol-containing phospholipids in Saccharomyces cerevisiae can be resolved by two-dimensional chromatography on silicic acid-impregnated paper. Four of these lipids are stable to mild alkaline methanolysis; one of these has already been characterized as a mannosyl di(inositolphosphoryl) ceramide. Addition of labeled 2-deoxy-d-glucose to a growing culture results in label appearing in five lipids, some of which are alkali-stable and have the same RF values as the alkali-stable inositol-containing phospholipids. These lipids are labeled rapidly. The deoxyglucose is incorporated intact, probably in a glycosidic link, since the deoxyglucose is released by mild acid treatment.Keywords
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