Interference by Cellular Melanin With Assay of DNA-Protein Crosslinks by the Potassium Dodecyl Sulfate Precipitation Method
- 1 April 1996
- journal article
- Published by Wiley in Pigment Cell Research
- Vol. 9 (2) , 68-71
- https://doi.org/10.1111/j.1600-0749.1996.tb00091.x
Abstract
The potassium dodecyl sulfate precipitation method was used to quantify DNA-protein crosslinks (DPCs) in lysates of melanoma cells exposed to ultraviolet radiation. Inducing melanin production in these cells before exposure to ultraviolet radiation decreased the apparent yield of DPCs. The decrease could also be produced by addition of melanin to lysates after exposure to crosslinking conditions. Experimental models could attribute this decrease to neither quenching of scintillations from the tritium label used nor to an effect of single strand breaks of DNA. This assay appears to be inappropriate for quantification of DPCs in melanized cells.Keywords
This publication has 3 references indexed in Scilit:
- A simple, sensitive assay to detect DNA–protein cromlinks in intact cells and in vivoCarcinogenesis: Integrative Cancer Research, 1992
- Eumelanin Causes DNA Strand Breaks and Kills CellsPigment Cell Research, 1987
- Ability of Melanins to Protect Against the Radiolysis of Thymine and ThymidinePigment Cell Research, 1987