lac Repressor blocks transcribing RNA polymerase and terminates transcription.
- 1 June 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (12) , 4134-4137
- https://doi.org/10.1073/pnas.83.12.4134
Abstract
Operator sequences are essential elements in many negatively controlled operons. By binding repressors, they prevent the formation of active complexes between RNA polymerase and promoters. Here we show that the Escherichia coli lac operator-repressor complex also efficiently interrupts ongoing transcription. This observation suggests a mechanism of action for operators located distal to promoter sequences.This publication has 18 references indexed in Scilit:
- Demonstration of two operator elements in gal: in vitro repressor binding studies.Proceedings of the National Academy of Sciences, 1984
- Cyclic AMP Receptor Protein: Role in Transcription ActivationScience, 1984
- Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymeraseProceedings of the National Academy of Sciences, 1983
- A control element within a structural gene: The gal operon of Escherichia coliCell, 1983
- Regulation of transcription from tandem and convergent promotersNucleic Acids Research, 1982
- Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.Proceedings of the National Academy of Sciences, 1981
- Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.Proceedings of the National Academy of Sciences, 1980
- Modulation of the two promoters of the galactose operon of Escherichia coliNature, 1979
- Genetic fusions defining trp and lac operon regulatory elementsJournal of Molecular Biology, 1975
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974