Rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction
- 1 December 1980
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 40 (6) , 1133-1135
- https://doi.org/10.1128/aem.40.6.1133-1135.1980
Abstract
An 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of MA-104 cells were treated with 50 microgram of trypsin per ml and hydroextracted overnight (4 degrees C) with polyethylene glycol 6,000. The concentrate was resuspended in 8 to 10 ml of tryptose phosphate broth and plaque assayed. Between 85 and 97% of the input virus could be recovered with a concentration of up to 15-fold.This publication has 15 references indexed in Scilit:
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