CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XIV. APPRAISAL OF ISOHEMAGGLUTININ ACTIVITY 1

Open Access
Publisher Website
Google Scholar
Abstract
Material rich in isohemagglutinins concentrated from pooled human blood plasma of appropriate blood group but unselected for titer was prepared by Pillemer et al. at the Harvard Pilot Plant and submitted to a group of consultants acting for the National Research Council, Subcommittee on Blood Substitutes. All 14 lots submitted were judged acceptably strong for use as blood grouping sera. The numerical titer and the speed of agglutination varied greatly as determined by the different workers. The variables were in the details of testing, and the sensitivity of test red blood cells from different sources. Certain lots of anti-A and anti-B material were prepared in large quantity, dried, and sealed in vacuo. These were accepted as "reference sera" with which comparisons could be made in the future. Thus the potency of a new serum can be detd. with respect to the reference serum using the same test cells. The sensitivity of red cells can also be tested against the reference serum.