Fluorescence Properties of Alkylated Guanine Derivatives.

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Abstract
The fluorescence excitation spectra of a number of quanine derivatives were characterized at 6 pH. Large differences were observed in the fluorescence intensities between the derivatives, although the fluorescence spectra were largely similar showing maxima at 290-295 nm when emission was at 400 nm. The fluorescence intensities correlated in part with the acid and alkaline dissociations of the molecules. The fluorescence spectra taken at different pH appears to be helpful in the identification of guanosine alkylation products such as 7-N-, 6-O- and 2-N-alkylguanosine. [Covalent modification of DNA by chemical carcinogens is thought to be an underlying mechanisms in the initiation of tumor growth. Most known carcinogens alkylate DNA bases, particularly guanine. Some of the established sites of alkylation are N7, O6, N2, Nl and C8 of guanine, depending on the type of carcinogen applied.].