Alterations in Hepatic Mitochondrial Function during Total Parenteral Nutrition in Immature Rats

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Abstract
To evaluate the effects of total parenteral nutrition (TPN) on hepatic mitochondrial function in immature rats, changes in hepatic energy charge levels and oxidative phosphorylation rates of hepatic mitochondria were studied along with the examination of serum chemical test. Male Wistar rats weighing 30 to 45 g were used and randomized into TPN (n = 8), enteral (n = 7), and control groups (n = 8). Parenteral and enteral groups were fed with TPN solution containing 19.3% dextrose, 3.19% amino acids, 1.05% fat emulsion, minerals and vitamins, and the control group with rat chow. The number of calories per kilogram per day was 550 × 1/4 on the 1st day, 550 × 1/2 on the 2nd, 550 × 3/4 on the 3rd, and 550 × 1 on the 4th day, based on the body weight on the 1st day. After the 5th day, 550 Kcal/kg/day was given, based on the body weight of the respective day. After 13‐day feeding, hepatic energy charge (EC), phosphorylation rate (PR) of hepatic mitochondria and serum chemical examination were carried out. EC was 0.871 ± 0.016 in the control group, 0.830 ± 0.019 in the enteral, and 0.785 ± 0.011 in TPN group (p < 0.001, compared with control group). PR was 138.9 ± 1.9, 133.0 ± 6.7, 111.0 ± 4.3, respectively, (p < 0.05, compared with control and enteral groups). There was no difference between the three groups on SGOT, SGPT, and total bilirubin. TPN group showed a deterioration of hepatic phosphorylation rate and energy charge in spite of normal serum transaminase levels. It is suspected that TPN has an adverse effect on hepatic mitochondrial function which may ultimately lead to hepatic dysfunction in infants. (Journal of Parenteral and Enteral Nutrition 14:640–645, 1990)

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