Highly Sensitive Bioluminescent Assay of Dehydrogenases using Nad (P) H: Fmn Oxidoreductase and Luciferase fromPhotoeacterium Fischeri
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 17 (18) , 2025-2034
- https://doi.org/10.1080/00032718408077195
Abstract
A highly sensitive bioluminescent assay of dehydrogenases was performed. NADH was produced by the catalytic action of alcohol and glucose-6-phosphate dehydrogenases and subsequently measured with high sensitivity by a bioluminescent assay using NAD (P) H : FMN oxidoreductase and luciferase from Photobacterium fischeri. The minimal amount of dehydrogenases that could be measured was 0.0055 amol (5.5 × 10−-21 mol).Keywords
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