Survey of T‐2 toxin in cereals by an indirect enzyme‐linked immunosorbent assay

Abstract
A survey of T‐2 toxin in cereals randomly sampled in 11 countries was carried out, employing an indirect competitive enzyme‐linked immunosorbent assay (ELISA). Among 540 samples, 72 (13%) contained over 10 ng/g of T‐2 toxin with an average of 91.5 ng/g. The content of T‐2 toxin was highest (500 ng/g) in an oat sample from Western Germany, and a relatively high content was observed in barley, wheat, oat and rye produced in Finland, Norway, Western Germany and the USSR. The ELISA method combined with a one‐step extraction procedure is proposed for the mass screening of T‐2 toxin in cereals.

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