Determination of captopril in blood and urine by high-performance liquid chromatography.
- 1 January 1981
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 29 (1) , 150-157
- https://doi.org/10.1248/cpb.29.150
Abstract
A new procedure was established for the determination of [the antihypertensive agent] captopril [1-(D-3-mercapto-2-methyl-1-oxopropyl)-L-proline] in biological fluids. Captopril was trapped with p-bromophenacyl bromide or N-(4-dimethylamino-3,5-dinitrophenyl)maleimide (DDPM), then the addition products were separated and determined by high-performance liquid chromatography (HPLC) on a reversed-phase column. The disulfides, metabolic products derived from captopril and excreted in the [human] urine, were reduced with tributylphosphine to captopril, which in turn was trapped with DDPM. These adducts were separated and determined by HPLC. The method provided quantitative results for captopril levels of 5-2000 ng/ml of whole blood and 0.1-100 .mu.g/ml of urine with satisfactory accuracy and percision.This publication has 6 references indexed in Scilit:
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