Synchronous and regulated expression of two AU-binding proteins, AUF1 and HuR, throughout murine development
- 9 November 2000
- journal article
- Published by Springer Nature in Oncogene
- Vol. 19 (47) , 5377-5384
- https://doi.org/10.1038/sj.onc.1203910
Abstract
The AUF1 (hnRNPD) and HuR (ELAV-like) proteins, potential trans-acting factors for regulated mRNA decay, bind in vitro to A+U-rich elements (AREs) found in the 3' untranslated region (3' UTR) of many labile transcripts. In an effort to determine whether these trans-acting factors are likely to play a role in embryogenesis, we have analysed their expression during mouse development both at the mRNA and protein levels. We show that AUF1 and HuR are expressed at all the developmental stages analysed from day 8.5 of embryonic development to adulthood. Expression levels are dynamic, varying between tissues and developmental stages. However, a strong positive correlation between AUF1 and HuR protein levels was observed in all examined tissues. Finally, we compared AUF1 and HuR expression with accumulation of one common target mRNA, c-myc. The similar spatio-temporal distribution of these proteins and of c-myc mRNA is in agreement with a potential concerted role in ARE-mediated control of mRNA stability.Keywords
This publication has 52 references indexed in Scilit:
- Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich elementGenes & Development, 1999
- Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteinsOncogene, 1998
- RNA stabilization by the AU-rich element binding protein, HuR, an ELAV proteinThe EMBO Journal, 1998
- AUF1 Binding Affinity to A+U-rich Elements Correlates with Rapid mRNA DegradationJournal of Biological Chemistry, 1996
- Regulation of the mRNA-binding Protein AUF1 by Activation of the β -Adrenergic Receptor Signal Transduction PathwayPublished by Elsevier ,1996
- Cloning and Characterization of HuR, a Ubiquitously Expressed Elav-like ProteinJournal of Biological Chemistry, 1996
- The UUAG-specific RNA Binding Protein, Heterogeneous Nuclear Ribonucleoprotein D0Published by Elsevier ,1995
- Glyceraldehyde-3-phosphate Dehydrogenase Selectively Binds AU-rich RNA in the NAD+-binding Region (Rossmann Fold)Journal of Biological Chemistry, 1995
- Identification of early neurogenic cells in the neural crest lineageDevelopmental Biology, 1992
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976