Composition and Characterization of tRNA from Methanococcus vannielii
- 1 January 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 133 (1) , 240-250
- https://doi.org/10.1128/jb.133.1.240-250.1978
Abstract
Purified bulk tRNA from M. vannielii (C source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by ion-exchange and TLC. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguanosine, N2,N2-dimethylguanosine, thiolated nucleosides, pseudouridine, dihydrouridine and O2''-methylcytidine were quantitated. In vitro methylation by M. vannielii extracts with S-adenosylmethionine and undermethylated E. coli tRNA revealed active tRNA methyltransferases for formation of methylated residues found in native M. vannielii tRNA, but none for the formation of 7-methylguanosine or ribothymidine. The native M. vannielii tRNA became methylated in the 7-methylguanosine position by E. coli extracts, but ribothymidine was not formed. Both M. vannielii and E. coli tRNA methyltransferases produced unidentified methylated residues in tRNA lacking or deficient in ribothymidine.This publication has 33 references indexed in Scilit:
- Base composition studies on mitochondrial 4 S RNA from rat liver and Morris hepatomas 5123D and 7777Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- 2'-O-Methylated oligonucleotides in ribosomal 18S and 28S RNA of a mouse hepatoma, MH 134Biochemistry, 1975
- Base analysis of RNA by 3H postlabeling — A study of ribothymidine content and degree of base methylation of 4 S RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Replacement of ribothymidine by 5‐methyl‐2‐thiouridine in sequence GTψC in tRNA of an extreme thermophileFEBS Letters, 1974
- Rapid and sensitive measurement of 7-methylguanosine and N6-isopentenyl derivatives of adenosine by cation-exchange chromatographyJournal of Chromatography A, 1971
- Primary sequence of tRNA1Val from Escherichia coli. B. I. Oligonucleotide sequences of digests of Escherichia coli tRNA1val with RNase T1 and pancreatic RNaseBiochemistry, 1971
- Nucleotide Sequences of Rat Liver Serine-tRNA. 1. Products of Digestion with Pancreatic RibonucleaseEuropean Journal of Biochemistry, 1971
- Restoration of aminoacylation activity of undermethylated transfer RNA by in vitro methylationBiochemical and Biophysical Research Communications, 1968
- Methane FermentationAnnual Review of Microbiology, 1967
- Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967