Implications of the Immunohistochemical Localization of the Carbonic Anhydrase Isozymes for Their Function in Normal and Pathologic Cellsa

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Abstract
Histochemical knowledge of the distribution of CA and the two isozymes CA I and CA II has been reviewed here. An abundance of CA occurs most commonly in epithelial cells specializing in transport of ions and water. A mechanism is favored whereby the polarity of efflux of CA-generated protons and bicarbonate across the apical versus the basolateral plasmalemma depends not on the location of CA, which is probably in the cytosol in most sites, but rather on the transport properties of the luminal compared with the serosal region of the plasma membrane in each epithelial cell type. CA exists also in some protein-secreting, merocrine cells including serous cells of salivary and tracheobronchial glands. Available evidence supports the possibility that CA stored as a secretory product in the cytoplasmic granules is released from these cells and, thus, implies extracellular biologic activity for CA in these sites. CA exists also in abundance in various nonepithelial cells performing different and not fully defined biologic functions in these cells. Prevalence of one isozyme over another varies in different cell types. A question remains whether the significance of this variability depends on work load or other undetermined factors.