Deletion of a DNA Polymerase β Gene Segment in T Cells Using Cell Type-Specific Gene Targeting
- 1 July 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5168) , 103-106
- https://doi.org/10.1126/science.8016642
Abstract
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.Keywords
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