Granulocyte-macrophage colony-stimulating factor does not increase the potency or efficacy of a foot-and-mouth disease virus subunit vaccine
Open Access
- 1 September 2005
- journal article
- Published by FapUNIFESP (SciELO) in Pesquisa Veterinária Brasileira
- Vol. 25 (3) , 150-158
- https://doi.org/10.1590/s0100-736x2005000300005
Abstract
Foot-and-mouth disease (FMD) is one of the most feared diseases of livestock worldwide. Vaccination has been a very effective weapon in controlling the disease, however a number of concerns with the current vaccine including the inability of approved diagnostic tests to reliably distinguish vaccinated from infected animals and the need for high containment facilities for vaccine production, have limited its use during outbreaks in countries previously free of the disease. A number of FMD vaccine candidates have been tested and a replication-defective human adenovirus type 5 (Ad5) vector containing the FMDV capsid (P1-2A) and 3C protease coding regions has been shown to completely protect pigs against challenge with the homologous virus (FMDV A12 and A24). An Ad5-P1-2A+3C vaccine for FMDV O1 Campos (Ad5-O1C), however, only induced a low FMDV-specific neutralizing antibody response in swine potency tests. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been successfully used to stimulate the immune response in vaccine formulations against a number of diseases, including HIV, hepatitis C and B. To attempt to improve the FMDV-specific immune response induced by Ad5-O1C, we inoculated swine with Ad5-O1C and an Ad5 vector containing the gene for porcine GM-CSF (pGM-CSF). However, in the conditions used in this trial, pGM-CSF did not improve the immune response to Ad5-O1C and adversely affected the level of protection of swine challenged with homologous FMDV.Keywords
This publication has 37 references indexed in Scilit:
- Novel Viral Disease Control Strategy: Adenovirus Expressing Alpha Interferon Rapidly Protects Swine from Foot-and-Mouth DiseaseJournal of Virology, 2003
- Induction of a protective response in swine vaccinated with DNA encoding foot-and-mouth disease virus empty capsid proteins and the 3D RNA polymeraseJournal of General Virology, 2001
- Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease VirusJournal of Virology, 2001
- Emergency vaccination against foot-and-mouth disease: Rate of development of immunity and its implications for the carrier stateVaccine, 1994
- Developments in foot-and-mouth disease vaccinesVaccine, 1991
- Use of peptides for immunization againast foot-and-mouth diseaseVaccine, 1988
- Aerobiology of foot and mouth disease: an outline and recent advancesRevue Scientifique et Technique de l'OIE, 1986
- Synthetic peptides mimic subtype specificity of foot-and-mouth disease virusFEBS Letters, 1983
- Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequenceNature, 1982
- The infectivity assay of foot-and-mouth disease virus in pigsEpidemiology and Infection, 1966