Flow cytometric measurement of DNA S‐phase in human bone marrow cells: correcting for peripheral blood contamination

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Abstract
Abstract:  The relationship between bone marrow (BM) cells with S‐phase DNA content and the amount of peripheral blood contamination estimated as percentage lymphocytes+monocytes (L+MO) present in BM samples has been investigated in a total of 136 BM aspirates and biopsy expellates from 35 hematologically healthy individuals. A significant negative correlation was demonstrated between total, erythroid and myeloid BM cells in S‐phase and the percentage of L+MO in the aspirates (r = 0.84, 0.57 and 0.49, respectively; p+ cells did not show any significant correlation between cells in S‐phase and percentage L+MO, indicating that peripheral blood contamination of BM aspirates is not a problem regarding kinetic investigations of these cells. In conclusion, the described flow cytometric method of analysing BM aspirates estimates the degree of peripheral blood contamination, as well as make possible a correct estimation of the DNA synthesis of several BM populations. The method is especially applicable when frequent BM sampling is required.

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