Administration of G‐CSF to healthy subjects: the effects on eosinophil counts and mobilization of eosinophil granule proteins

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Abstract
Any influence of G-CSF on eosinophils is mostly negative, although reports which have studied this relationship are few with varied results. The aim of this study was to investigate the influence of G-CSF administration to healthy subjects on eosinophils in peripheral blood. Blood eosinophil counts, serum levels of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and eosinophil protein X (EPX), as well as cell morphology were studied. 14 healthy volunteers received 7.5 μg (n = 8) or 10 μg/kg body weight (n = 6) G-CSF daily for six consecutive days. ECP and EPX were assessed by specific RIAs and EPO by a specific FEIA. Cell morphology was examined by electron microscopy. During G-CSF administration, eosinophil counts increased from 0.22 ±0.04 ×109/l to 0.61 ± 0.098 × 109/l (P = 0.001), serum ECP from 12.39 ± 2.45 μg/l to 61.82 ± 7.38 μg/l (P = 0.0014), serum EPX from 28.05 ±4.54 μg/l to 87.96 ±9.84 μg/l (P =0.002) and serum EPO from 8.89 ±2.2 μg/l to 19.98 ± 5.1 μg/l (P = 0.003). All variables returned gradually to initial values after discontinuation of G-CSF. Distinct changes in the morphology of secondary granules were observed 24 h after G-CSF administration. The granules became irregular and their matrix less electron dense. We conclude that administration of G-CSF to healthy humans increases the number of circulating eosinophils and affects the mobilization of eosinophil granule proteins.

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