The sss gene product, which affects pyoverdin production in Pseudomonas aeruginosa 7NSK2, is a site‐specific recombinease
- 1 December 1994
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 14 (5) , 1011-1020
- https://doi.org/10.1111/j.1365-2958.1994.tb01335.x
Abstract
Pyoverdin production by Pseudomonas aeruginosa strain 7NSK2 was induced by Zn(II) in the presence of iron. A mutant was isolated in which Zn(II) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases. An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF. The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coii xerC mutant and the E. coii xerC gene could complement a genomic sss mutation in P. aeruginosa.Keywords
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