The mechanism by which epidermal growth factor inhibits glycogen synthase kinase 3 in A431 cells
- 1 October 1994
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 303 (1) , 27-31
- https://doi.org/10.1042/bj3030027
Abstract
Glycogen synthase kinase 3 (GSK3) was inhibited by 50% within 5 min when A431 cells were stimulated with epidermal growth factor (EGF). The inhibition was unaffected by rapamycin at concentrations which blocked the activation of p70 S6 kinase, and reversed by incubation with protein phosphatase-1. EGF stimulation of A431 cells inhibited GSK3 alpha and GSK3 beta to a similar extent, and inhibition was accompanied by phosphorylation of the tryptic peptides containing the serine residues phosphorylated in vitro by p70 S6 kinase or MAP kinase-activated protein (MAPKAP) kinase-1 beta (also termed Rsk-2). These results demonstrate that EGF inhibits GSK3 by inducing phosphorylation of a serine residue and that GSK3 is not phosphorylated in vivo by either p70 S6 kinase or protein kinase C.Keywords
This publication has 17 references indexed in Scilit:
- The α‐isoform of glycogen synthase kinase‐3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase‐activated protein kinase‐1 in vitroFEBS Letters, 1994
- Inactivation of glycogen synthase kinase-3β by phosphorylation: new kinase connections in insulin and growth-factor signallingBiochemical Journal, 1993
- Inhibitor‐2 functions like a chaperone to fold three expressed isoforms of mammalian protein phosphatase‐1 into a conformation with the specificity and regulatory properties of the native enzymeEuropean Journal of Biochemistry, 1993
- Drosophila shaggy kinase and rat glycogen synthase kinase-3 have conserved activities and act downstream of NotchNature, 1993
- A Specific Immunoprecipitation Assay for the Protein Kinase FA/Glycogen Synthase Kinase 3Analytical Biochemistry, 1993
- Glycogen synthase kinase-3: functions in oncogenesis and developmentBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1992
- Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinasesCell, 1992
- Putative protein kinase product of the Drosophila segment-polarity gene zeste-white3Nature, 1990
- [37] Protein phosphatase-1 and protein phosphatase-2A from rabbit skeletal musclePublished by Elsevier ,1988
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976