Site-Directed ELISA Identifies a Highly Antigenic Region of the Simian Immunodeficiency Virus Transmembrane Glycoprotein
- 1 January 1988
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 4 (3) , 159-164
- https://doi.org/10.1089/aid.1988.4.159
Abstract
The transmembrane glycoprotein (gp32) of the simian immunodeficiency virus (SIV) contains a highly antigenic region that includes amino acid residues 606-628. A synthetic peptide representing this region was highly immunoreactive with sera from SIV-infected primates in a site-directed enzyme-linked immunosorbent assay (ELISA). This reactivity extended across four primate species from three genera and identified infection with at least two distinct isolates of SIV. This site-directed ELISA represents a simple, accessible method with broad specificity for screening large numbers of primates for antibodies against SIV.Keywords
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