Histomathematical Analysis of Clinical Specimens: Challenges and Progress
Open Access
- 1 February 2005
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 53 (2) , 177-185
- https://doi.org/10.1369/jhc.4a6457.2005
Abstract
Proteomic analysis of clinical tissue specimens is a difficult undertaking. Described here is a multiplex study of protein expression levels in histological sections of human prostate that addresses many of the associated challenges. Whole-mount sections from 10 prostatectomy specimens were studied using 15 antibodies, immunohistochemical staining, digital imaging, and mathematical analysis of the data sets. The approach was successful in stratifying cell lineages present in the samples based on proteomic patterns, including differentiating normal epithelium from cancer. This strategy likely will be a useful method for extending the number of proteins that can be analyzed in clinical cancer specimens using currently available laboratory techniques.Keywords
This publication has 18 references indexed in Scilit:
- Phenotypic composition of salivary gland tumors: an application of principle component analysis to tissue microarray dataLaboratory Investigation, 2004
- Computer based receptogram approach: an objective way of assessing immunohistochemistry of androgen receptor staining and its correlation with hormonal response in metastatic carcinoma of prostateJournal of Clinical Pathology, 2004
- Increased risk of malignant progression in benign proliferating breast lesions defined by expression of heat shock protein 27British Journal of Cancer, 2004
- Expression study of three secretory proteins (prostatic secretory protein of 94 amino acids, probasin, and seminal vesicle secretion II) in dysplastic and neoplastic rat prostatesThe Prostate, 2003
- Fas and Fas ligand expression is elevated in prostatic intraepithelial neoplasia and prostatic adenocarcinomaCancer, 2002
- Evaluation of Non-Formalin Tissue Fixation for Molecular Profiling StudiesThe American Journal of Pathology, 2002
- Validation of image analysis for enzyme histochemical and immunocytochemical stainingBiotechnic & Histochemistry, 2002
- Delineation of prognostic biomarkers in prostate cancerNature, 2001
- Image analysis and quantification in lung tissueClinical and Experimental Allergy, 2001
- Sensitive Immunoassay of Tissue Cell Proteins Procured by Laser Capture MicrodissectionThe American Journal of Pathology, 2000