Ultra-Sensitive Immunoassays Using Multi-Photon-Detection in Diagnostic Proteomics of Blood
- 18 November 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 4 (6) , 2375-2378
- https://doi.org/10.1021/pr050320n
Abstract
Multiphoton-detection methods that detect as little as 1000 atoms of 125I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10−50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFα, VEGF, IL-1β, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics. Keywords: multiphoton-detection • blood • serum • proteomics • diagnostics • immunoassay • cytokines • growth factors • breast cancer • HIV-1Keywords
This publication has 4 references indexed in Scilit:
- Ultra-high sensitivity multi-photon detection imaging in proteomics analysesProteomics, 2005
- Serum Proteomics in Cancer Diagnosis and ManagementAnnual Review of Medicine, 2004
- Perspectives for mass spectrometry and functional proteomicsMass Spectrometry Reviews, 2001
- PSA as a Marker for Prostate Cancer: A Critical ReviewAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1996