Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3

Open Access

31 January 1986

journal article
research article
Published by American Society for Microbiology in Journal of Bacteriology

Vol. 165 (2) , 448-452
https://doi.org/10.1128/jb.165.2.448-452.1986

Abstract

D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.

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