Loss of transforming activity of plasmid DNA (pBR322) in E. coli caused by singlet molecular oxygen
- 19 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 211 (1) , 49-52
- https://doi.org/10.1016/0014-5793(87)81272-3
Abstract
Plasmid DNA pBR322 in aqueous solution was exposed to singlet molecular oxygen (1O2) generated by microwave discharge. DNA damage was detected as loss of transforming activity of pBR322 in E.coli (CMK) dependent on the time of exposure. DNA damage was effectively decreased by singlet-oxygen quenchers such as sodium azide and methionine. Replacement of water in the incubation buffer by D2O led to an increase in DNA damage. 9,10-Bis(2-ethylene)anthracene disulfate was used as a chemical trap for 1O2 quantitation by HPLC analysis of the endoperoxide formed.Keywords
This publication has 13 references indexed in Scilit:
- Dna Damage by Chemically Generated Singlet OxygenFree Radical Research Communications, 1987
- Phototoxicity of the tetracyclines: photosensitized emission of singlet delta dioxygen.Proceedings of the National Academy of Sciences, 1986
- Inability of Chemically Generated Singlet Oxygen to Break the DNA BackboneFree Radical Research Communications, 1985
- Radiolysis of DNA and Model Systems in the Presence of OxygenPublished by Elsevier ,1985
- Oxygen-independent direct deoxyribonucleic acid backbone breakage caused by Rose Bengal and visible lightJournal of Photochemistry, 1984
- Singlet Oxygen Formation Detected by Low-Level Chemiluminescence during Enzymatic Reduction of Prostaglandin G2to H2Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Molybdenum catalysts for allylic alkylationJournal of the American Chemical Society, 1982
- Reactivity of purine and pyrimidine bases toward singlet oxygenBiophysical Journal, 1971
- Reactions between Singlet Oxygen and the Constituents of Nucleic AcidsBiophysical Journal, 1970
- The dye-sensitized photooxidation of purine and pyrimidine derivativesArchives of Biochemistry and Biophysics, 1964