Transport of Nucleic Acid Bases into Escherichia coli

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Abstract
The uptake and retention of purine bases and of uracil requires a protonmotive force and intracellular conversion to nucleotides. Inhibition of uptake by arsenate does not imply that ATP is required for the transport process because arsenate caused a rapid fall in the level of 5-phosphoribosyl 1-pyrophosphate. A mutation defective in high-affinity adenine transport was identified and is designated purP. This mutation maps near mtl. Competition experiments indicate that at least 2 other systems are used to transport quanine, xanthine and hypoxanthine.