Oligodendrocyte cell surface recognized by a novel monoclonal antibody specific to sulfatide

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Abstract
A RAT monoclonal antibody (OL-1) was obtained by in-vitro immunization of rat spleenocytes with para-formaldehyde-fixed primary cultured glial cells derived from newborn rat brain and subsequent fusion with a rat myeloma cell line. The antibody secreted by the hybridoma immunostains live rat and mouse oligo-dendrocytes in primary and secondary cultures. The antibody binds specifically to oligodendrocytes and myelin structures in-situ. Radioimmunolabelling assays with a number of purified glycolipids offer thin layer chromatography separation show that OL-1 antibody binds strongly to sulfatide and to a lesser extent to galactosyl diglyceride.

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