Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test

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Abstract
Highly repeated sequences of nuclear DNA from barley Hordeum vulgare (L.) variety ‘Erfa’ were cloned. Several clones containing barley specific repeated DNA were analysed by sequence analysis and Southern blot hybridization. The investigated repeats differ from each other in their length, sequence and redundancy. Their length ranges from 36 bp to about 180 bp. The repeats are AT-rich and differ widely in their redundancy within the barley genome. Southern analysis showed that the repeats belong to different repetition complexes. The possibility for utilizing these clones as probes for simple and fast genome analysis is demonstrated in squash dot experiments.