Influence of Selective Light Scattering on Measurements of Absorption Spectra of Chlorella.

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Abstract
By varying (1) the refractive index of the suspension medium, and (2) the geometry of the optical system of the spectrophotometer, there were obtained 2 sets of absorption curves which revealed different effects of scattering. The 1st set were extinction curves of cells in various solutions of bovine serum albumen (0-40% albumen). The 2nd set was obtained by allowing the detector to receive only light coming from the suspension at specified angles (between 0[degree] and 22[degree] to the incident beam). The curves show large apparent changes in the shapes and positions of absorption bands both with the medium and with angle. Controls indicate that these effects are optical in nature. The 1st series of experimental curves are in good agreement with predictions of the Mie theory of scattering.