Double‐ and single‐strand conformation polymorphism analysis of point mutations and short tandem repeats
- 1 January 1994
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 15 (1) , 566-571
- https://doi.org/10.1002/elps.1150150177
Abstract
Single point mutations in small DNA fragments and single unit differences in simple repetitive DNA can be detected as double‐strand conformation polymorphisms using polyacrylamide gel electrophoresis, with and without sodium dodecyl sulfate, even at temperatures as low as 3°C. Changes in a single base are distinguished by means of the analysis of the heteroduplexes, and changes in more than two bases can be distinguished in both homoduplexes and heteroduplexes. Polymerase chain reaction (PCR) conditions can be designed not only to amplify homoduplexes, heteroduplexes and singlestrand DNA at the same time, but also to focus the analysis on either singlestrand conformation polymorphism (SSCP) or double‐strand conformation polymorphism (DSCP). DSCP seems to be advantageous in typing DNA polymorphisms or mutations in loci with few variants, but, because it is necessary to have a simple pattern of all possible combinations of the alleles, it is not as advantageous in typing systems with many variants.Keywords
This publication has 22 references indexed in Scilit:
- Rapid detection of single base mismatches as heteroduplexes on Hydrolink gelsTrends in Genetics, 1991
- Electrophoretic human leukocyte antigen HLA‐DQA1 DNA typing after polymerase chain reaction amplificationElectrophoresis, 1991
- Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reactionGenomics, 1989
- Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.Proceedings of the National Academy of Sciences, 1989
- Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.Proceedings of the National Academy of Sciences, 1989
- Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.Proceedings of the National Academy of Sciences, 1989
- Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresisNucleic Acids Research, 1985
- Detection of single base substitutions in total genomic DNANature, 1985
- Sequence-Determined DNA SeparationsAnnual Review of Biophysics and Bioengineering, 1984
- DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.Proceedings of the National Academy of Sciences, 1983