High-speed Liquid Chromatographic Analysis of Drugs. II : Rapid Estimation of Berberine in Crude Drugs
Open Access
- 1 January 1977
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 97 (8) , 940-943
- https://doi.org/10.1248/yakushi1947.97.8_940
Abstract
A rapid estimation procedure using a high-speed liquid chromatography is described for determination of berberine in crude drugs (Phellodendron cortex, Coptis rhizome and Phellodendron extract). Separation can be achieved within 10 min employing a 1 m column packed with Zipax SCX, using 0.1 M NaClO4 (CH3CN-H2O 6:4) and 0.2 M H3BO3-0.002 M NaClO4 (pH 8.5) (7:3) solution as an eluant in a Du Pont LC 830 liquid chromatograph. Berberine in crude drugs was extracted with citric acid-methanol solution and injected into the column. Accuracy in this method apparently was less than .+-. 1% with the relation Y = 13.806 X + 0.0025, where X = quantity of berberine (.mu.g) and Y = peak height of berberine (cm) and a relative coefficient of 0.999.Keywords
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