In vitro incorporation of selenium into tRNAs of Salmonella typhimurium.
Open Access
- 1 August 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (16) , 6341-6344
- https://doi.org/10.1073/pnas.87.16.6341
Abstract
Broken-cell preparations of Salmonella typhimurium rapidly incorporated 75Se from 75SeO3(2-) into tRNA by an ATP-dependent process. Selenium incorporation in the presence of 50 microM 75SeO3(2-) (0.8-1 pmol per A260 unit) was enhanced by the selenocysteine precursor, O-acetyl-L-serine (to 3.7 pmol per A260 unit). This increase in incorporation was a function of O-acetyl-L-serine concentration. Neither O-acetyl-L-homoserine nor O-phospho-L-serine stimulated the incorporation of selenium into tRNA. The incorporation of 75Se from 75SeO3(2-) was decreased by adding L-selenocysteine but not by adding the D isomer. When homologous bulk tRNA was added to the broken-cell preparations, an increased rate of 75Se labeling was observed. The supernatant fraction of the broken-cell preparation contained all of the enzymes required for this process. Reversed-phase HPLC analysis of labeled bulk tRNA digested to nucleosides showed the presence of a labeled compound that coeluted with authentic 5-methylaminomethyl-2-selenouridine.This publication has 30 references indexed in Scilit:
- Distribution of two selenonucleosides among the selenium-containing tRNAs from Methanococcus vannieliiProceedings of the National Academy of Sciences, 1984
- Quantitative enzymatic hydrolysis of tRNAsJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue.Journal of Biological Chemistry, 1982
- Selenium-containing tRNAGlu from Clostridium sticklandii: correlation of aminoacylation with selenium content.Proceedings of the National Academy of Sciences, 1982
- Selenium-containing tRNAs from Clostridium sticklandii: cochromatography of one species with L-prolyl-tRNA.Proceedings of the National Academy of Sciences, 1980
- Occurrence of selenocysteine in the selenium-dependent formate dehydrogenase of Methanococcus vannieliiArchives of Biochemistry and Biophysics, 1979
- Some Selenium‐Dependent Biochemical ProcessesPublished by Wiley ,1979
- Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteineBiochemistry, 1978
- Chemical characterization of the selenoprotein component of clostridial glycine reductase: identification of selenocysteine as the organoselenium moiety.Proceedings of the National Academy of Sciences, 1976
- Primary structure of tRNALysof E. coli BNucleic Acids Research, 1975