The promotion of secondary structures in single-stranded DNA by drugs that bind to duplex DNA: an atomic force microscopy study
- 12 August 2008
- journal article
- Published by IOP Publishing in Nanotechnology
- Vol. 19 (38) , 384016
- https://doi.org/10.1088/0957-4484/19/38/384016
Abstract
We study the behavior of single-stranded DNA (ssDNA) in the presence of well-known drugs with either an intercalating binding mode, such as daunorubicin, actinomycin D, and chloroquine, or a minor groove binding mode, such as netropsin and berenil, by atomic force microscopy (AFM). At very low salt conditions, ssDNA molecules adopt an unstructured conformation without secondary structures. We observe that under these conditions additions of drugs that bind to double-stranded DNA (dsDNA) promote the formation of secondary structures in ssDNA. Furthermore, with an increase of concentration of the drugs, the extension as well as the thermal stabilization of these hairpins was observed.Keywords
This publication has 38 references indexed in Scilit:
- DNA and its associated processes as targets for cancer therapyNature Reviews Cancer, 2002
- DNA-Binding of Drugs Used in Medicinal TherapiesCurrent Medicinal Chemistry, 2002
- Intercalative Binding of Small Molecules to Nucleic AcidsCurrent Organic Chemistry, 2000
- Drug-DNA recognition: energetics and implications for designJournal of Molecular Recognition, 2000
- Recent developments in sequence selective minor groove DNA effectors.Current Medicinal Chemistry, 2000
- Crystal structure of a double-stranded DNA containing a cisplatin interstrand cross-link at 1.63 A resolution: Hydration at the platinated siteNucleic Acids Research, 1999
- NMR Solution Structure of a DNA Dodecamer Duplex Containing a cis-Diammineplatinum(II) d(GpG) Intrastrand Cross-Link, the Major Adduct of the Anticancer Drug CisplatinBiochemistry, 1998
- Energetics of drug–DNA interactionsBiopolymers, 1997
- Visualizing Protein-Nucleic Acid Interactions on a Large Scale with the Scanning Force MicroscopeAnnual Review of Biophysics, 1996
- DNA sequence preferences of several AT-selective minor groove binding ligandsNucleic Acids Research, 1995