Technique for Human Bone Marrow Harvest

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Abstract
The usual technique of harvesting bone marrow for allogeneic or autologous transplantation involves passage of the marrow suspension through discs of stainless steel mesh of increasingly small diameter. A sterile technique which is much less messy and produces a single cell suspension is described. Potter-Elvehjem homogenizers are used to break up the marrow particles. This procedure was used successfully in 6 patients where allogeneic transplantation was performed and in 6 harvests of autologous marrow. Marrow cryopreserved in this way contains viable committed stem cells and is not subject to agglutination following subsequent thawing.

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