COMPARISONS OF COMPLEMENT-FIXATION AND INDIRECT FLUORESCENT-ANTIBODY REACTIONS IN DETECTION OF BOVINE BABESIASIS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (2) , 153-156
Abstract
Complement-fixation (CF) and indirect fluorescent antibody (IFA) antigens were prepared from Babesia bigemina isolates obtained in Texas [USA]. These serologic procedures were evaluated on 130 serum samples sequentially collected from 5 B. bigemina-infected mature cattle, beginning on the day of exposure and continuing for 175 days thereafter. Both tests were effective in detecting specific antibodies for the first 84 days of infection, with 57 of 60 (95%) sera tested being positive on the CF test and 57 of 57 (100%) tests being positive to the IFA test. From 98-175 days, 24 of 60 (40%) of the sera tested were positive with the CF test, and 53 of 56 (95%) were positive with the IFA test. During the first 84 days, a similar linear regression occurred in CF and IFA serum titers, but after 98 days the IFA regression flattened out; the CF titers decreased below the sensitivity threshold in 60% of the sera tested.

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