Mixed Agglutination with Platelets

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Abstract
Platelet layers firmly adhering to the glass surface were obtained by allowing human platelets to sediment onto the wall of test tubes or onto glass slides and ‘fixing’ them by means of a 2% formaldehyde solution. Mixed agglutination tests with these platelet preparations were performed in following the procedure used for studies on monolayer cell cultures. It was shown that platelets contain human species-specific antigens similar to those present on all human nucleated cells. Reactions with human sera containing antibodies to alloantigens were also studied. Mixed agglutination tests with platelets gave antiserum titers 100 times higher than those obtained in the corresponding lymphocytotoxicity tests. In some instances, mixed agglutination tests with platelets detected HL-A antigens which were not demonstrable by means of lymphocytotoxicity tests.
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