Leucine Synthesis in Spinach Chloroplasts: Partial Characterization of 2-Isopropylmalate Synthase

1 January 1993

journal article
Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler

Vol. 374 (7-12) , 1105-1108
https://doi.org/10.1515/bchm3.1993.374.7-12.1105

Abstract

Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The Km values were 5 microM for acetyl-CoA and 75 microM for 2-oxoisovalerate.

Keywords

SPINACH CHLOROPLASTS
PARTIAL CHARACTERIZATION
ISOPROPYLMALATE SYNTHASE
LEUCINE SYNTHESIS
SYNTHESIS IN SPINACH

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