Activity of β-galactosidase in homogenates and isolated microvilli fraction of jejunal mucosa from suckling rats
- 1 August 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 96 (2) , 492-494
- https://doi.org/10.1042/bj0960492
Abstract
[beta]-Galactosidase activity was studied in homogenates and isolated microvilli fraction of jejunal mucosa from 14-day-old suckling rats. o-Nitrophenyl [beta]-D-galactoside served as the substrate. The microvilli fraction contains about one-third of the total activity of the orginal homogenate. The pH optimum of the [beta]-galactosidase was 3.5 in the total homogenate and supernatant fraction, whereas in the microvilli fraction the maximum activity was at pH 5[middot]5. This work gives further support to the view that two [beta]-galactosidases exist in the jejunal mucosa.This publication has 5 references indexed in Scilit:
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