Direct evaluation of fluorescence in single renal epithelial cells using a mitochondrial probe (DASPMI)
- 1 November 1983
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 132 (2) , 143-148
- https://doi.org/10.1111/j.1365-2818.1983.tb04265.x
Abstract
The study of distribution and quantitation of a fluorescent probe in living epithelia with the aid of an inverted microscope requires that individual cells can be analyzed without optical interference from adjacent cells. The application of fluorescence microscopy and fluorometry to a recently developed in vitro culture system of renal epithelial cells was described. Epithelial cells derived from the mammalian renal cortical collecting tubule (CT) and the thick ascending loop of Henle (TAL) are cultivated as continuous monolayers in serum-free, hormone-supplemented media. A specific mitochondrial marker (DASPMI) is added to the medium and incoporated into the cytoplasm. The microscopic image reveals that the mitochondrial fluroescence distribution differs between CT and TAL cultures. The fluorometric quantitation shows a normally distributed histogram of medium-range intensity in TAL cell cultures while CT cultures exhibit a 2-peak pattern of mitochondrial fluorescence distribution among epithelial cells.Keywords
This publication has 9 references indexed in Scilit:
- Differential response to hormones of defined distal nephron epithelia in cultureAmerican Journal of Physiology-Cell Physiology, 1983
- Nephron epithelia in culture: Growth of loop of Henle cells in hormonally defined serum-free mediaCell Biology International Reports, 1981
- Hormonal stimulation and differential growth response of renal epithelial cells cultivated in vitro from individual nephron segmentsInternational Journal of Biochemistry, 1980
- Application of differential interference contrast with inverted microscopes to the in vitro perfused nephronJournal of Microscopy, 1979
- Primary culture of mammalian nephron epitheliaPflügers Archiv - European Journal of Physiology, 1979
- Morphology of rabbit collecting ductJournal of Anatomy, 1979
- Structural-functional heterogeneity along the rabbit collecting tubuleKidney International, 1978
- Dimethylaminostyrylmethylpyridiniumiodine (DASPMI) as a fluorescent probe for mitochondria in situBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976