A RADIOIMMUNOASSAY FOR MEASUREMENT OF 3,3'-L-DIIODOTHYRONINE (T2)
- 1 September 1976
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 43 (3) , 682-685
- https://doi.org/10.1210/jcem-43-3-682
Abstract
A sensitive, specific and reproducible radioimmunoassay CRIA) for measurement of 3,3'-L-diiodothyronine (T2) in concentrated ethanol extracts of serum and amniotic fluid is described. The T2 binding antiserum was prepared by immunization of rabbits with T2-human serum albumin conjugate. Of various thyroid hormone derivatives tested, only 3-L-monoiodothyronine cross-reacted significantly with T2 binding sites on the antiserum. The mean serum T2 concentration (ng/100 ml) was 7.6 in normal subjects, 20 in hyperthyroidism, 6.0 in hypothyroidism, 7.3 in pregnancy, 7.3 in cirrhosis and 16 in newborn cord blood. T2 concentration in amniotic fluid at term (11 ng/100 ml) was higher than that (4.1 ng/100 ml) at 15–16 wk. of gestation. A significant increase in serum T2 was detected following ingestion of T3 and rT3. These data suggest that: (a) T2 is a normal component of human serum and amniotic fluid; (b) peripheral metabolism of T3 as well as rT3 contribute significantly to serum T2 in man.Keywords
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