Characterization of a Human Monoclonal Antibody to Lipopolysaccharides of Pseudomonas aeruginosa Serotype 5: A Possible Candidate as an Immunotherapeutic Agent for Infections with P. aeruginosa
- 1 November 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 152 (5) , 965-970
- https://doi.org/10.1093/infdis/152.5.965
Abstract
A human monoclonal antibody, P3D9 (lgG2, γ type), that bound to Pseudomonas aeruginosa Homma serotype 5 cells with high specificity was produced by cell fusion between a human tonsillar lymphocyte and a mouse plasmacytoma cell, P3-X63-Ag8-U1 (P3U1). The yield of P3D9 secreted into the culture supernatant from the mouse-human hybridoma was 2–10 µg/ml, and this hybridoma cell line has been stably producing this antibody for six months. Antibody P3D9 alone did not cause agglutination of serotype 5 cells, but the cells were agglutinated after addition of goat antibody to human IgO. Antibody P3D9 was proven to have protective activity against infection with P. aeruginosa, and the 50% protective dose estimated for experimental peritoneal infection with P. aeruginosa was 2.4 µg per mouse.Keywords
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