Abstract
Developing muscles from forelegs of 11- to 18-day-old mouse embryos were stained in situ for cholinesterase with the Cu-ferrocyanide technique. The skin of the legs represented a diffusion barrier for the incubation medium. In older embryos the skin was mechanically removed after trypsin digestion. In younger embryos the skin remained on the forelegs after trypsin treatment. With this technique it was possible to follow the establishment of the muscular pattern in the legs.

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