IGE AND IGG ANTIBODY AGAINST HUMAN (RECOMBINANT DNA) INSULIN IN PATIENTS WITH SYSTEMIC INSULIN ALLERGY

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 105  (1) , 108-113
Abstract
IgE and IgG antibodies to human (rDNA) insulin as well as to bovine and porcine insulin were demonstrated in the serum of 2 patients with systemic insulin allergy by an enzyme-linked immunosorbent assay. IgE and total antibody binding to bovine insulin was demonstrated with the use of radioimmunoassays. Both patients had cutaneous reactivity to all 3 insulins. When the serum of 1 patient was preincubated with human, porcine or bovine insulin, there was inhibition of binding of the patient''s IgE and IgG antibodies to human insulin. The other patient had very low levels of IgE antibodies to insulin and thus only IgG inhibition was possible. Preincubation with human insulin inhibited binding of each patient''s antibodies to bovine or porcine insulin. For these 2 patients, human insulin has all the antigenic determinants that bovine and porcine insulin have. Human insulin for these 2 patients will not eliminate insulin allergy in all patients with systemic allergy to animal insulin, because there are patients whose antibodies recognize determinants common to commercial human, bovine and porcine insulin.