The formation of dehydroalanine residues in alkali-treated insulin and oxidized glutathione. A nuclear-magnetic-resonance study

Abstract
1H- and 13C-NMR measurements enable direct observation of the rate of formation of dehydroalanine residues resulting from lysis of the S-S bonds of [pig] insulin and oxidized glutathione in base at pD 13. The data provide clear evidence for the .beta.-elimination mechanism for this reaction. The dehydroalanine-containing products from the lysis of insulin undergo secondary reactions.