RADIORECEPTOR ASSAY FOR INSULIN
- 1 February 1976
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 42 (2) , 399-402
- https://doi.org/10.1210/jcem-42-2-399
Abstract
A sensitive and simple radioreceptor assay (RRA) capable of detecting 10 μU/ml of serum insulin has been developed using the crude membrane fraction of guinea pig kidney and 125I-porcine insulin. Using this assay system, serum insulin during 100 g oral glucose tolerance tests in six males including one obese man was measured. The mean concentration of serum insulin increased from 19 μU/ml during fasting to maximum of 90 μU/ml after 60 min of glucose load and then gradually declined to 38 μU/ml by 180 min. The values obtained by the present RRA agreed well with immunoreactive insulin (IRI).Keywords
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