Escherichia coli strains in which chromosome replication is controlled by a P1 or F replicon integrated into oriC
- 1 June 1996
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 20 (5) , 1013-1023
- https://doi.org/10.1111/j.1365-2958.1996.tb02542.x
Abstract
We report the construction of intP1 and intFs strains, in which the basic replicon from either plasmid P1 or plasmid F (oriS) has been integrated in both orientations into the origin of replication, oriC, of the Escherichia coli chromosome. In these strains, oriC is no longer functional and chromosome‐replication is instead controlled by the integrated plasmid replicon. The strains were viable, showing that the deviation from normal chromosome‐replication control was not large enough to prohibit cell survival. The strains showed a broader cell‐size distribution than a wild‐type strain and were more filamentous in rich than in minimal media, although cells of wild‐type size were also present. Cells which contained aberrantly shaped or aberrantly distributed nucleoids were also observed. Marker‐frequency analysis indicated that chromosome replication was predominantly bidirectional in both intFs strains. In the intP1 strains, the degree of bidirectionality depended upon the orientation of the integrated replicon.Keywords
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