HISTOCHEMICAL STUDY ON GLYCOGEN SYNTHESIS FROM GLUCOSE-1-PHOSPHATE IN BLOOD CELLS UNDER NORMAL AND PATHOLOGICAL CONDITIONS

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Abstract
Polysaccharides of glycogen class were histochemically synthesized from glucose-1-phosphate at pH 5.7 in various blood and bone marrow cells except the crythrocytic series. The intense activity of phosphorylase was indicated blue with iodine which stained a long straight chain of 1,4-linkages, while the branching enzyme (amylo-1,4 → 1,6-transglucosidase) activity was indicated red violet by staining shorter chains of a branched polysaccharide. These reactions appeared most frequently in neutrophilic, pseudoeosinophilic or amphophil series, particularly in the mature cells of the series. The histochemical synthesis of glycogen from glucose-1-phosphate in neutrophil leucocytes in the peripheral blood varies under pathologic conditions.