Correlations between densitometry of red-free photographs and reflectometry with the scanning laser ophthalmoscope in normal subjects and glaucoma patients

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Abstract
Objective: To compare image measurements of the retina produced by scanning laser ophthalmoscopy with those from red-free photographs (RFP) of the nerve fibre layer.Subjects: The left eyes of 23 subjects (10 normal, 7 glaucoma suspect and 6 glaucomatous) were included in this study.Method: All the eyes were photographed using standard red-free photography, and images of the retina were digitised directly from the SLO.Results: The correlation coefficient for all except three subjects was > 0.45, and the probability (p) that R=0 was < 0.05 in all but 5 eyes.Conclusions: The data indicate that variations in retinal surface reflectivity as measured by the SLO are similar to those recorded on negative film during RFP. This further suggests that the SLO can be useful for making objective measurements of the RNFL, without the intermediate and variable steps of photography.